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thp1 dualtm cells thpd nfis (InvivoGen)

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InvivoGen thp1 dualtm cells thpd nfis

a, immune cell types in the TME identified through scRNAseq of CD45 + cells isolated from orthotopically implanted M3-9-M OVA cells; b, number of immune cell types detected in M3-9-M OVA tumours through scRNAseq experiment (n = 2 pooled samples per experimental groups); c, number of significantly impacted genes obtained from pairwise comparisons (Wilcoxon rank sum test); d, circos plot of all significantly impacted genes, obtained from pairwise comparisons of all cell types measured, that overlap with each other through identical or shared pathways; e, bar graph of transcriptional regulators of 3,2-HPP impacted genes; f-m, NF-κB and IRF induction in mouse RAW-Dual™ cells and human <t>THP1-Dual™</t> cells with different pathogen recognition receptor agonists in the presence of HPP metabolites (HI = heat inactivated, three combined experiments, error bars represent standard error, one-way ANOVA test).

Thp1 Dualtm Cells Thpd Nfis, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells+thpd+nfis/THP1-Dual+Cells/bio_rxiv__64898__2026__04__23__720410-176-3-11
Average 97 stars, based on 569 article reviews

thp1 dualtm cells thpd nfis - by Bioz Stars, 2026-09

97/100 stars

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1) Product Images from "Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells"

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

Journal: bioRxiv

doi: 10.64898/2026.04.23.720410

Figure Legend Snippet: a, immune cell types in the TME identified through scRNAseq of CD45 + cells isolated from orthotopically implanted M3-9-M OVA cells; b, number of immune cell types detected in M3-9-M OVA tumours through scRNAseq experiment (n = 2 pooled samples per experimental groups); c, number of significantly impacted genes obtained from pairwise comparisons (Wilcoxon rank sum test); d, circos plot of all significantly impacted genes, obtained from pairwise comparisons of all cell types measured, that overlap with each other through identical or shared pathways; e, bar graph of transcriptional regulators of 3,2-HPP impacted genes; f-m, NF-κB and IRF induction in mouse RAW-Dual™ cells and human THP1-Dual™ cells with different pathogen recognition receptor agonists in the presence of HPP metabolites (HI = heat inactivated, three combined experiments, error bars represent standard error, one-way ANOVA test).

Techniques Used: Isolation

a, NF-κB activity in THP1-Dual™ cells treated with LPS in the presence of vehicle or HPP metabolites for 0-24 hours (two combined experiments, error bars represent standard error); b-e, NF-κB or IRF activity in THP1-Dual™ cells treated with different inducers in the presence or absence of HPP isomers for 16 hours (three combined experiments, error bars represent standard error, one-way ANOVA test).

Figure Legend Snippet: a, NF-κB activity in THP1-Dual™ cells treated with LPS in the presence of vehicle or HPP metabolites for 0-24 hours (two combined experiments, error bars represent standard error); b-e, NF-κB or IRF activity in THP1-Dual™ cells treated with different inducers in the presence or absence of HPP isomers for 16 hours (three combined experiments, error bars represent standard error, one-way ANOVA test).

Techniques Used: Activity Assay

a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

Figure Legend Snippet: a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

Techniques Used: Comparison, Cell Culture, Western Blot

a, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells treated with LPS±HPPs for 16 hours, measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); b, LDH release from THP1-WT vs. – GSDMD -KO cells treated with LPS±HPP for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test); c-d, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells © and human PBMCs (d) treated with LPS and NG±HPPs for 16 hours. measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); e-f, LDH release from THP1-WT vs. – GSDMD -KO cells € and human PBMCs (f) treated with LPS and NG±HPPs for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test).

Figure Legend Snippet: a, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells treated with LPS±HPPs for 16 hours, measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); b, LDH release from THP1-WT vs. – GSDMD -KO cells treated with LPS±HPP for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test); c-d, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells © and human PBMCs (d) treated with LPS and NG±HPPs for 16 hours. measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); e-f, LDH release from THP1-WT vs. – GSDMD -KO cells € and human PBMCs (f) treated with LPS and NG±HPPs for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test).

Techniques Used: Activity Assay

Cell Culture:

Article Title: Surface immunogenic protein from Streptococcus agalactiae and Fissurella latimarginata hemocyanin are TLR4 ligands and activate MyD88- and TRIF dependent signaling pathways.
Article Snippet: .. THP-1 Dual cells (InvivoGen, thpd-nfis), TRIF KO Dual Reporter THP1 Cells (InvivoGen, thpd-kotrif), THP1-DualTM KO-MyD cells (InvivoGen, thpd-komyd), and TLR4 KO Dual Reporter THP- 1 Cells (InvivoGen thpd-koTLR4) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco), L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin (Gibco), 100 mg/mL normocin (InvivoGen, ant-nr-1), 100 mg/mL zeocin (InvivoGen, antzn-1), and 10 mg/mL blasticidin (InvivoGen, ant-bl-1). ..

Article Title: Immunotherapy with engineered bacteria by targeting the STING pathway for anti-tumor immunity
Article Snippet: The Prism7.0 c software (GraphPad Software, San Diego, CA) was used for all statistical analysis. .. THP1-Dual cells (thpd-nfis) containing an IRF-luciferase reporter and NF-κB-alkaline phosphatase (SEAP) reporter, THP1-Dual KO-cGAS (thpd-kocgas) cells with the stable knockout of the cGAS gene and THP1-Dual KO-STING (thpd-kostg) cells with the stable knockout of the STING gene were purchased from InVivoGen and cultured following the manufacturers protocols. .. THP-1 cells lines containing the following TMEM173 (STING) alleles were utilized: H232 (InVivoGen, thpd-h232), HAQ-THP1 endogenous allele (InVivoGen, thpd-nfis) and R232 (InVivoGen, thpd-r232).

other:

Article Title: Discovery of isoindoline-2(1H)-carboxamide STING inhibitors as anti-inflammatory agents.
Article Snippet: 1 Small-Molecule Drug Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People’s Republic of China 2 State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People’s Republic of China 3 Lingang Laboratory, Shanghai 200031, People’s Republic of China 4 School of Physical Science & Technology, ShanghaiTech University, Shanghai 201210, People’s Republic of China 5 University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China 6 Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264117, Shandong, People’s Republic of China Abstract STING (stimulator of interferon genes) is an endoplasmic reticulum-resident membrane-spanning protein that is widely expressed in mammalian cells and functions as a central regulator for the innate immunity.. Aberrant activation of the STING axis due to loss-of-function or gain-of-function mutation leads to various autoimmune and autoinflammatory disorders such as Aicardi-Goutières syndrome, systemic lupus erythematosus, and STING-associated vasculopathy with onset in infancy.. Here we report the design, synthesis, and structure–activity relationship (SAR) of the isoindoline-2(1H)carboxamide STING inhibitors.

Article Title: Tau activation of microglial cGAS–IFN reduces MEF2C-mediated cognitive resilience
Article Snippet: THP1-Dual cells (thpd-nfis) and THP1-Dual KO-cGAS cells (thpd-kocgas) were purchased from InvivoGen.

Article Title: Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience.
Article Snippet: THP1-Dual cells (thpd-nfis) and THP1-Dual KO-cGAS cells (thpd-kocgas) were purchased from InvivoGen.

Article Title: Discovery of indole derivatives as STING degraders.
Article Snippet: Aberrant activation of the stimulator of interferon genes (STING) pathway is associated with the development of various inflammatory and autoimmune diseases.. Targeting STING for degradation represents a novel strategy for the treatment of these diseases.. In this study, we designed and synthesized a series of STING-PROTACs based on a nitro-free covalent warhead and different E3 ligase binders.

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Journal: bioRxiv

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

doi: 10.64898/2026.04.23.720410

Figure Lengend Snippet: a, immune cell types in the TME identified through scRNAseq of CD45 + cells isolated from orthotopically implanted M3-9-M OVA cells; b, number of immune cell types detected in M3-9-M OVA tumours through scRNAseq experiment (n = 2 pooled samples per experimental groups); c, number of significantly impacted genes obtained from pairwise comparisons (Wilcoxon rank sum test); d, circos plot of all significantly impacted genes, obtained from pairwise comparisons of all cell types measured, that overlap with each other through identical or shared pathways; e, bar graph of transcriptional regulators of 3,2-HPP impacted genes; f-m, NF-κB and IRF induction in mouse RAW-Dual™ cells and human THP1-Dual™ cells with different pathogen recognition receptor agonists in the presence of HPP metabolites (HI = heat inactivated, three combined experiments, error bars represent standard error, one-way ANOVA test).

Article Snippet: RAW-DualTM cells (rawd-ismip), THP1-DualTM cells (thpd-nfis), HEK-Blue TM IL-1β cells (hkb-il1bv2; InvivoGen), THP1-Null2 Cells (thp-nullz) and THP1-KO-GSDMD cells (thp-kogsdmdz) were obtained from InvivoGen (San Diego, CA, USA).

Techniques: Isolation

Journal: bioRxiv

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

doi: 10.64898/2026.04.23.720410

Figure Lengend Snippet: a, NF-κB activity in THP1-Dual™ cells treated with LPS in the presence of vehicle or HPP metabolites for 0-24 hours (two combined experiments, error bars represent standard error); b-e, NF-κB or IRF activity in THP1-Dual™ cells treated with different inducers in the presence or absence of HPP isomers for 16 hours (three combined experiments, error bars represent standard error, one-way ANOVA test).

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

doi: 10.64898/2026.04.23.720410

Figure Lengend Snippet: a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

Techniques: Comparison, Cell Culture, Western Blot

Journal: bioRxiv

Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

doi: 10.64898/2026.04.23.720410

Figure Lengend Snippet: a, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells treated with LPS±HPPs for 16 hours, measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); b, LDH release from THP1-WT vs. – GSDMD -KO cells treated with LPS±HPP for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test); c-d, IL-1β activity in the supernatant of THP1-WT vs. - GSDMD -KO cells © and human PBMCs (d) treated with LPS and NG±HPPs for 16 hours. measured using HEK-Blue™ IL-1β reporter cells (three combined experiments, error bars represent standard error, one-way ANOVA with Bonferroni test); e-f, LDH release from THP1-WT vs. – GSDMD -KO cells € and human PBMCs (f) treated with LPS and NG±HPPs for 16 hours (three combined experiments, one-way ANOVA with Bonferroni test).

Techniques: Activity Assay

Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

Journal: Materials Today Bio

Article Title: Polyelectrolyte nanoparticles enable intracellular delivery of STING protein fragments for ovarian cancer immunotherapy

doi: 10.1016/j.mtbio.2026.103251

Figure Lengend Snippet: Polyanion component of nanoparticle allows for tuning cell specificity in STING activation. ( A ) Chemical structures of polyanions examined in nanoparticle formulations. ( B ) Z-Average diameter and polydispersity index (N = 8-13 independent formulations) as well as ( C ) zeta potential (N = 8-10 independent formulations) measurements of nanoparticles. (D) Fold-change in STING signaling activation compared to buffer 24 h after treatment with nanoparticle formulations at STINGΔTM doses of 5 μg/mL (RAW264.7), 8 μg/mL (HEK293T), 10 μg/mL (THP1), or 20 μg/mL (BPPNM and SKOV3). STING signaling measured as luminescent IRF3 reporter for RAW264.7, THP1, and HEK293T reporters or chemokine CXCL10 secretion in supernatant for SKOV3 and BPPNM. Each row represents one of N = 3 biological replicates, displaying the geometric mean of N = 3 technical replicates measuring the fold change in STING activation compared to the buffer control. Data represented as geometric mean ± standard deviation for data on log-scale and mean ± standard deviation for data on linear scale.

Article Snippet: RAW-Lucia ISG (rawl-isg), THP1-Dual (thpd-nfis), and HEK-Blue mTLR4 (hkb-mtlr4) reporter cells were purchased from InvivoGen.

Techniques: Activation Assay, Zeta Potential Analyzer, Control, Standard Deviation

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thp1 dualtm cells thpd nfis (InvivoGen)

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1) Product Images from "Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells"

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